College supplement essay
Sunday, November 3, 2019
Accidents and Catastrophes Essay Example | Topics and Well Written Essays - 2500 words
Accidents and Catastrophes - Essay Example , meaning depending on the number of casualties and destruction done or according to the nature of the accident, that is whether it was caused by human or natural factors (Quarantelli, 1987). The linear model of classifying accidents uses ââ¬Ësizeââ¬â¢ to categorize and give different names to accidents (UN, 1992). An emergency can be termed as being an unanticipated occurrence that threatens life and property and calls upon immediate and urgent reaction from the relevant authorities within the given social set up. It is to be found that each social set up has its own way of coping with emergencies and, that there are different pre-arranged response depending on the magnitude of the emergency situation (Porfiriev, 1995). A disaster is an occurrence that causes extensive damage to the infrastructure within a given societal set up that is so severe to the extent that the resources available within the society itself are not adequate to function a response. At the same time a disaster jars the day to day activities of the society (Porfiriev, 1995). While a disaster might disrupt the functioning within a social set-up, a catastrophe brings a society to a standstill. A catastrophe throws a community into total mayhem where institutions of leadership may completely disintegrate and other institutions like schools and hospitals may be wholly destroyed. Catastrophes call for international intervention if the society affected is so crippled that it does not have the capacity to help its own survivors and casualties (Quarantelli, 1987). Although there is no unanimous agreement as to how accidents are to be listed using the linear model, it is generally accepted that, from the smallest to the most dire the order is an incident emergency, disaster and catastrophe. There are other terminologies that occur frequently when dealing accidents and their nature. A calamity is an occurrence that, like a disaster brings about large-scale disruptions in the normal functions of a
Friday, November 1, 2019
Management situation in which you are to apply a prominent motivation Essay
Management situation in which you are to apply a prominent motivation theory to improve performance - Essay Example This theory posits that every individual has specific motivational needs, which are the driving factors for their efforts. Vroom (1964) explains this model of motivation (M) based on three components namely, Valence, Instrumentality and Expectancy. The Expectancy theory or the VIE theory states that motivation is a product of valence, instrumentality and expectancy (M=VxIxE). Valence (V) refers to the value of an outcome that an individual places on various outcomes of their jobs like pay, incentives, interest in the work, relationships, etc. If an individual values relationships, then he/she would be happy to work where relationships are strong and valued. Instrumentality (I) refers to an employeeââ¬â¢s opinion or belief about the extent of efforts required to achieve an outcome. Employees tend to put in efforts to perform a specific job only if they believe that their efforts would result in outcomes they value. The third component, Expectancy (E) refers to the employeesââ¬â¢ perception about the extent to which his/her effort will result in certain level of job performance. Expectancy refers to level of confidence individuals possess about a specific action or job (George & Jones, 2008). These components have a strong relationship between each other and on overall motivation. The relationship between effort and performance is referred to as the E-P linkage, which is determined by expectancy. Instrumentality refers to the relationship between performance and outcome, referred to as the P-O linkage. Any limitation in these linkages or in the valence significantly impacts individualsââ¬â¢ motivation because motivation is a product of these three components (Isaac et al, 2001). In the present case two issues have been identified: one is related to the expectancy and the other is about instrumentality. The expectancy of few employees is low as evident from their low confidence because of knowledge and information related issues associated with the new pr ocess. The valence of this group lies in abilities, knowledge and skills. In this case, as suggested by Isaac et al., (2001) the supervisor must diagnose the membersââ¬â¢ abilities and their perceptions related to the new process. Secondly, the supervisor needs to train, coach and mentor the members on the new process until they achieve the desired outcomes. The supervisor needs to spend more time with the team and encourage every achievement. Appreciating individuals that achieve the desired outcomes will improve their confidence because the appreciation will act as recognition to their valences. This will slowly improve their confidence and strengthen their expectancy and performance linkage. The supervisor needs to constantly provide support when team members are facing issues in achieving the desired outcomes; moreover, the leader needs to provide constant feedback to the team members in a way that their mistakes are being corrected and their self-confidence is also not being disturbed. Another motivational issue in the present case is with instrumentality, which impacts the P-O linkage. A few members that valued pay were not motivated to work because they believed that the amount of effort required to produce the specific outcome is not worth the pay they were getting. In such situation, the supervisor should talk to the individuals and explain the situation with
Wednesday, October 30, 2019
Case analysis Montego Bay Essay Example | Topics and Well Written Essays - 750 words
Case analysis Montego Bay - Essay Example In order to protect her store and keep her employees motivated, she agrees with Theresa Daley, operations director for Montego Bay, to test a new computerized scheduling system, which is expected to reduce payroll costs and improve productivity. Mahoney learns that the new system has several benefits; however, she is also weary that this system will disrupt the collegial and productive work environment she had created in the store. A careful examination of the businessââ¬â¢ status shows that Mahoney, as a manager, has failed to create and maintain a working environment that motivates her employees. Motivation amongst employees improves the quality and productivity of work, since employees are triggered to work towards achieving a common goal. This paper describes how Mahoney can create a working environment that will motivate her employees by examining some theoretical perspectives on motivating employees. The Maslowââ¬â¢s hierarchy of needs is one of the theories that managers apply to create a working environment that motivates workers. This theory describes how people fulfill a range of personal needs in the context of their work. Maslow postulated that there is a common pattern of needs identification and satisfaction, which many people follow in the same sequence (Kaufman, 2004). According to this theory, a person has to satisfy the low-level needs (physiological and safety) before he or she pursues upper-level needs (social, esteem and self-actualization). Most employees usually have problems articulating what they want from a job. Mangers should, therefore, offer different incentives to workers so as to help them identify and satisfy each need in turn, and progress up the hierarchy. Mahoney, as the store manager, should recognize that her employees have different needs, and not all are motivated in the same way, and all do not move up the hierarchy at the same pace (Kaufman, 2004). Her duty is to adapt and change her style to fit the employeesâ⬠⢠needs, other than forcing them into something they are not. In addition, she has to test different techniques with every employee in order to determine which ones are considered effective, and which ones are not. The employees should be encouraged to follow their own self interests, which motivate them to pursue higher level needs and improve the quality of work. The workers should be motivated to higher levels of individual commitment and personal expectations. As an employee progresses though an organization, his employer should supply opportunities to satisfy high level needs on Maslowââ¬â¢s pyramid (Montana & Charnov, 2008). Another important theory of workerââ¬â¢s motivation is Herzbergââ¬â¢s motivator ââ¬âHygiene theory. Herzberg suggested hygiene factors, which do not create or motivate satisfaction and ââ¬Å"motivatorsâ⬠. These include interpersonal relations, salary, supervision, working condition, and company policy (Montana & Charnov, 2008). Accordi ng to this theory, absence of dissatisfies can create job dissatisfaction, but their presence does not create or motivate satisfaction. Herzberg, therefore, established from a data that motivators are elements that enrich a personââ¬â¢s job. He came up with five motivators, which were strong determiners of job satisfaction: responsibility, achievement, advancement, recognition and the work itself. The hygiene factors constantly produce only short-term changes in job performance and attitude,
Monday, October 28, 2019
Henrik Ibsens A Dolls House Essay Example for Free
Henrik Ibsens A Dolls House Essay The role, treatment, disadvantages, and sacrifices of women in their societies are vital themes in both Federico LOrcas The House Of Bernarda Alba and Henrik Ibsens A Dolls House. In Federico LOrcas The House Of Bernarda Alba, to start with, all characters that appear on stage are females, and men are merely talked about. In Ibsens A Dolls House the protagonist is Nora, a woman who gets treated by her husband as though she were a little girl. At the time when Federico LOrca and Henrik Ibsen wrote their plays, it was normal for women to get treated as anything but equal to men. Women were expected by men and by their society to do nothing more than stay at home, cook for their family, get children, and then take care of their children as well. Although Ibsen and LOrca wrote these plays several decades apart, since Ibsen wrote his play at the turn of the century in Norway and LOrca wrote his in 1930s Spain, the expectations of women were pretty much the same. It was not normal for women to receive an education, let alone a good one, and women were not allowed to vote. In A Dolls House there are three female characters: Nora, Mrs. Linde, and the maid working for Nora. All of these women have to sacrifice something and have disadvantages, simply because they are women. Every character in The House Of Bernarda Alba has to suffer because they are women. Spanish tradition in the 1930s forces them all to isolate themselves from the outer world for eight years of their lives. In The House Of Bernarda Alba, Bernarda Alba and all of her daughters have to mourn the death of their father. This is Spanish tradition, and so they all have to live in their house for eight years, completely isolated from the rest of society. They may only wear black, even in the scorching heat of southern Spain. This already shows how harshly women were treated. Bernarda Alba and her daughters are forced to isolate themselves from the outside world completely, whether they want to or not, because of tradition. The eight women are forced to give up eight years of their lives in which they may do nothing but mourn. Also, the fact that a woman has to kill her own child when she gets pregnant, and is then hunted down by the whole town and killed herself, shows how women were viewed and treated. Also, a woman could often not decide who she wanted to marry, and when a women got married it was often just nothing more than a business deal, and the feelings of the woman were completely ignored.
Saturday, October 26, 2019
energy :: essays research papers
à ·Ã à à à à There are 5 forms of energy: mechanical, chemical, radiant, electrical, and nuclear à ·Ã à à à à Mechanical energy is the form of energy of familiar objects and machines. à ·Ã à à à à Chemical energy is the form of energy involved in chemical reactions. Chemical energy is released in the chemical reaction known as oxidation. à ·Ã à à à à Radiant energy is energy that travels through space. Radiant energy includes light and all other parts of the electromagnetic spectrum. à ·Ã à à à à Electrical energy is another form of energy from electromagnetic interactions that will be considered in detail later. à ·Ã à à à à Nuclear energy is a form of energy often discussed because of its use as an energy source in power plants. Nuclear energy is another form of energy from the atom, but this time the energy involves the nucleus, the innermost part of an atom, and nuclear interactions. à ·Ã à à à à Any form of energy can be converted to another form. A light bulb, for example, converts electrical energy to radiant energy. à ·Ã à à à à The law of conservation of energy: Energy is never created or destroyed. Energy can be converted from on form to another but the total energy remains constant. à ·Ã à à à à Energy arrives from the sun, goes though a number of conversions, and then radiates back into space. The total sum leaving eventually equals the original amount that arrived. à ·Ã à à à à Work is the product of an applied force and the distance through which the force acts. Work is measured in Newton-meters, a metric unit called a joule. Power is work per unit of time. Power is measured in watts. One watt is 1 joule per second. Power is also measured in horsepower. One horsepower is 550 feet 1lb/sec. à ·Ã à à à à Energy is the ability to do work. An object that is elevated against gravity has a potential to do work. The object is said to have potential energy, or energy of position. Moving objects have the ability to do work on other objects because of their motion. The energy of motion is called kinetic energy.
Thursday, October 24, 2019
Religious Language and Concepts in Romeo and Juliet
Religious Language and Concepts in Romeo and Juliet The epistle of Saint John unequivocally states, ââ¬Å"Love comes from Godâ⬠(1 John 4:7). This statement not only explains the source of love but it also provides a means to understand both love and God. If love is from God, then an understanding of love can be derived from knowing God. Thus, the converse, knowing love provides a level of knowledge concerning God, is true. In light of this conclusion, it only seems natural that the two should intersect when trying to describe one another. William Shakespeare employs Christian language and concepts in the play Romeo and Juliet to not only effectively conveys the gravity of love but also to provide metaphorical undertones to the playââ¬â¢s conclusion. It is apparent that Shakespeare intentionally used religious language and concepts in order to elicit the implications that are attached with the words. By glossing over these words as two-dimensional adjectives much of Shakespeareââ¬â¢s beauty and genius is lost and the intrinsic harmony connecting love and God is unknown to the reader. The play Romeo and Juliet is steeped in religious language and constructions. The possible examples are numerous and wide ranging, but some are used to convey love while others are used to drive the thematic plot. For organizational purposes, the usages of religious language that help convey the meaning of love will be addressed first followed by an explication of the thematic usages or religious language. An excellent example of how Shakespeare implements religious language and concepts in order to describe the transcendent emotion of Love is in Romeo and Julietââ¬â¢s first meeting. While courting Juliet, Romeo says, ââ¬Å"My lips, two blushing pilgrims, ready stand, To smooth that rough touch with a tender kiss. â⬠(1:5:97-98) Prior to this statement Romeo had equated Juliet with a holy shrine and he then employs the religious concept of pilgrimage in the following lines. On a very surface level, this makes sense considering that a holy shrine is an end goal and pilgrims, like lips moving in for a kiss, travel to the end goal. However, it seems rather evident that Shakespeare meant much more than simply making a comparison for movement in this statement. The term pilgrim calls to mind the departure from a known place into an unknown, holy land for the sake of obtaining salvation. By using ââ¬Ëpilgrimââ¬â¢ to describe the kiss shared between the two lovers implies that Romeo and Juliet are going to depart from their current love-starved world and move into a holy world of love. Another example of where Shakespeare implements religious language is when Romeo says, ââ¬Å"I take thee at thy word: Call me but love, and Iââ¬â¢ll be new baptized; henceforth I never will be Romeo. (2:2:49-51) Again, as in the previous statement, Shakespeare implemented religious language in order to describe how love is a transcendent and unearthly entity. In the realm of Christianity, Baptism is the sacramental shedding of earthly imperfections and wedding of the soul to Heavenââ¬â¢s dominion. Romeoââ¬â¢s statement uses the word and concept of baptism to express that by being called Julietââ¬â¢s lover, Romeo would shed his earthly self an d enter the world of love where his name would no longer matter. Both of these statements allow Shakespeare to describe the transcendence of love, and logically the only way to describe the transcendence of love is by implementing religious words and concepts that are themselves transcendent. It is rather evident why Shakespeare employed religious language instead of using secular or earthly language and concepts. As stated previously, both love and God are entities that find their origin outside of earthly confines. The fact that both love and God manifest themselves on earth creates a dilemma when one attempts to describe their essence. Trying to describe God or love with words that are limited to earthââ¬â¢s confines is similar to the proverbial square peg in a round hole. This is why it would not be conducive for Shakespeare to describe love with secular or earthly words. The inadequate secular language would lose much of loveââ¬â¢s weight and Shakespeareââ¬â¢s genius would be repressed. Shakespeareââ¬â¢s usage of religious language not only allows for better description of love itself but Shakespeare also uses it as a vehicle for metaphor. The central message of Christianity is the redemptive sacrifice of the ââ¬Ëunblemished lambââ¬â¢, Jesus Christ, known as the Gospel. When Romeo kisses Juliet and says, ââ¬Å"Thus from my lips, by yours, my sins are purged. â⬠(1:5:109) The purging of sins inevitably draws up thoughts about the Gospel within the readerââ¬â¢s mind and although the metaphor is not brought to denouement within just this one line, the groundwork is set out. Later in the play, Juliet says in regards to performing her mock-death, ââ¬Å"Things that, to hear them told, have made me tremble; And I will do it with out fear or doubt, To live an unstained wife to my sweet love. â⬠(4:1:86-88) This line again is drenched in Gospel metaphor. The word ââ¬Ëunstainedââ¬â¢ is a queue for the reader that this line is not merely a secular, two-dimensional statement and with this in mind, Juliet seems to share much of Jesus Christââ¬â¢s emotions in the biblical account of Him praying in the Garden of Gethsemane. Both Jesus and Juliet are apprehensive of their looming deaths, both admit that they are afraid, and both choose to confront their fears with confidence. With both this line and the previously stated line it is relatively clear that Shakespeare created certain parallels between Juliet and Jesus Christ. These parallels come to an ultimate conclusion at the playââ¬â¢s conclusion. At the end of scene five, when both Romeo and Juliet are dead, it becomes evident that Shakespeareââ¬â¢s metaphor of Christ has come to conclusion. After both of the families realize that their respective children are dead they give up their long held resentment towards each other. This reconciliation seems to echo the reconciliation found after Jesus Christââ¬â¢s death. Obviously, in no way is Juliet an airtight allegory for the Gospel. However, these statements and constructions are undeniable in their intentional resemblance to Christ and the Gospel story. Shakespeare manipulating his plot to facilitate the Gospel metaphor implies that he felt strongly about the need to use God to describe love. Jesus Christ came from heaven and through His death brought salvation for the sinful world. Juliet embodied love and through her death brought reconciliation to the town of Verona. Shakespeare, through his metaphors, is attempting to convey a very weighty assessment on love. The metaphor conveys that love is not of this world but instead from God and thus to know either God or love is to know something of both. It also suggests that love has a very real salvation within it, the ability to reconcile relationships and transcend earthly pettiness. It seems ironic that despite the fact that God created the world, worldly terms fall short of describing His essence. Likewise, it is equally ironic that love, an entity that seemingly controls the vast majority of all human interactions in one way or another, is not readily described by commonplace terms. Juxtaposing these two ironies makes it evident as to why William Shakespeare implemented religious terminology and metaphors in order to fully convey the essence of love. Romeo and Juliet were undoubtedly in love with each other and it is fitting that their holy love could not be constrained by either the unholy confines of Verona or of secular diction.
Wednesday, October 23, 2019
Literature Review for Denaturing Gradient Gel Electrophoresis
Denaturing Gradient Gel Electrophoresis (DGGE) is a robust process by which point mutation can be detected. It depends upon polymerase chain reaction (PCR) products which denature at different temperatures depending upon if they contain homoduplex or different products from both wild type and mutated genes or heteroduplex or combined strains of wild type and mutated DNA strains. Heteroduplex products contain a mismatch and therefore melt more quickly than heteroduplex products (Roelfsema and Peters, 2005, pp. 79).DGGE is used most effectively to identify point mutations in genomic DNA that cause genetic diseases, to identifiy previously unidentified mutations in recessive genes, analysis of DNA from cancer tumors, and by targeting RNA, assessing the number and type of bacteria species in soil, water, and the human body (ibid, pp. 84). The DGGE process has many complicated steps that entail trial and error starting with designing the proper Gradient Gel so that the PCR product will en ter one end as a double strand, quickly denature and then stop progressing and stick in place.First the PCR product must be designed using special software that analyses the melting curves of possible PCR products. In order to keep the DNA stuck in place once the denaturing has occurred, a GC clamp consisting of a string of 40-60 nucleotides must be attached to the PCR primer resulting in a high denaturing temperature at one end and not the other. In addition, the Gradient gel must be prepared properly with a 30% gradient and adjusted so that the DNA gets trapped directly in the middle (ibid, 80).Finally, in the most technically difficult step, a constant temperature of 60 degrees Celsius must be achieved in which to perform the electrophoresis. After this the gels are soaked in a 0. 5XTAE containing ethidium bromide to visualize the DNA. Once these steps are completed successfully, the results are very clear, and seen quickly. If large numbers of samples have to be screened, DGGE i s very reliable and cost effective(ibid, 85). The following is a summary of some of the recent uses of DGGE and findings of researchers who have been exploring new territory in their fields through the use of DGGE.This review aims to highlight the strengths and weaknesses of DGGE and its most effective applications. In a study led by J. Walter of the University of Stuttegart in Germany, 16S rhibosomal primers were also used to detect lactic acid bacteria in human faeces. Subjects were given the probiotic strain lactobacillus rhamnosus DR20 to drink, and while cultural methods only detected the strain in one of the subjects, DGGE detected it in both.In addition, the food associated samples did not appear in the rosa agar cultures, but were apparent in the DGGE profile (Walter, J et al, 2001). This shows the sensitivity and reliability of DGGE methods, and its applicability to human microbiology. Another study of faeces conducted by Maukonen et al. concluded that DGGE was an effective way to establish the stability of certain groups of gastrointestinal bacteria. They successfully established the stability and diversity of the Erecta group by using DGGE to study the bacteria from 12 subjects (Maukonen et al, 2002).Applications to the study of animal faeces uncovered that DGGE is effective in identifying complex systems such as Heliobacters which are difficult to culture (Al-Soud et al, 2003). At the University of Wales in Cardiff, a team of researchers led by Charlotte E. Davies compared bacterial microfloras of healing and nonhealing chronic venous leg ulcers using both cultural and 16S rhibosomal PCR-DGGE methods. PCR-DGGE analysis found a much higher load of pseudonomads in nonhealing wounds than was apparent in cultural analysis alone (Davies, C et al, 2003).This proves the applicability of DGGE to human microbiology and its usefulness in identifying causes of illness. Similar results were obtained in studying Hypophatasia at the Barnes-Jewish Hospital Resear ch Institute. DGGE was used to identify mutations in severely affected patients and was found 100% effective in identifying recessive mutations. In addition it identified eight new mutations and one new polymorphism of hypophatasia confirming its genotypic variability (Mumm, S et al, 2002).DGGE can therefore be a powerful tool in diagnosing hypophatasia and other genetic diseases. Italian biologists used 16S rDNA PCR-DGGE and ISR-PCR methods as tools to differentiate between strains of staphyloccus isolated from fermented sausages. They obtained species specific profiles using DGGE and combining the two methods allowed them to identify 10 species and an additional 7 groups. They concluded that combining the two microbial techniques was what led to their success (Blaiotta, G et al, sep 2003).Corroborating this evidence of the need to use DGGE in combination with other techniques when studying food microbiology, a study of Cassava root fermentation in Brazzaville, Congo determined tha t the most effective method of isolating and identifying microbial communities in Cassava starch fermentation was to combine culture and DGGE methods. They found that DGGE failed to detect pure cultures recovered from enrichment and yet detected other species not apparent in any of the cultural methods used (Miambi, E, Guyote, JP and Ampe, F. , 2003).These results suggest that DGGE, while reliable and sensitive, is dependant upon other methods to complete a profile of the microbiological communities. Strides have been made to understand and identify the ecology of microbial communities, such as the work done at the University of Nottingham. Researchers used PCR-DGGE analysis focused on the V3 and V4-V5 regions of 16S genes to identify and lactobacillus and Staphlyococcus bacteria in stilton cheese. They then used Florescence in Situ Hybridization (FISH) experiments to identify the spatial arrangement of microbial species in the dairy matrix.This allowed them to conclude that there a re specific ecological reasons for microbial growth in cheese, and that there are real applications of the combination of DGGE and FISH to optimize food fermentation and preservation of traditional products (Ercolini, Hill, Dodd, Jul 2001). DGGE also has applications to farm production as seen in de Olivera et alââ¬â¢s study of soil rhibosomes, which concluded that DGGE provides fingerprinting of rhibosomes useful in determining the effect of agricultural practices on soils.This can help in the proper amendment of soils and monitoring of pesticides (de Olivera et al, 2006). Recognizing the usefulness of DGGE in identifying and categorizing microbial communities, and the need for more effective identification of which DNA regions to study, Zhongtang Yu and Mark Morrison performed a test to compare DGGE profiles across hypervariable (V) regions taken from the same DNA regions, and identify the most useful V regions to study in gastrointestinal microbiomes.Their recommendation is th at amplification of the V3 or V1 regions of rrs genes gives the best result, but when doing a longer amplification, the V3 to V5 or V6 to V8 range should be targeted (Yu and Morrison, 2004). DGGE has been used successfully in oceanography to identify and isolate protists that are so small they lack taxonomic features and are too unstable to be studied by traditional means.Biologists at the Woods Hole Oceanographic Institution studied protistan assemblages from the Antarctic using DGGE and were able to determine that microenvironments significantly impact assemblages and that significant genetic diversity exists in each microenvironment (Gast, Dennett and Caron, 2004). Building on the sensitivity of DGGE in identifying genetic differences, biologists in Germany identified an entirely new phylogenic group of Eukariyotic bacteria in the deeper layers of tidal flats. Their technique included using primers targeted at the 18S rRNA gene.They were also able to establish distant relationshi ps between Eukaria and grazers and deposit feeders, proving DGGEââ¬â¢s applicability to taxonomy. Scientists at the University of Montana also recognized DGGEââ¬â¢s usefulness in identifying unculturable communities and developed a way to make these communities more visible by DGGE. They first put the communities through GC fractionalization to make the study size smaller which allowed previously undetectable or underrepresented bands from the full community analysis to be seen (Holbien et al, 2004).Seeking to improve the sensitivity and versatility of DGGEââ¬â¢s application to microbial ecology as well as provide a way to compare and standardize gradient gels, Neufeld and Mohn of the University of British Columbia tested the use of Fluorophore-Labled primers. They found that fluorophore greatly helped intralane normalization, was relatively cheap, and allows DGGE versatility including running RNA and DNA derived patterns in the same lane (Neufeld and Mohn, 2005). References Al-Soud, Waleed Abu; Bennedsen, Mads; On, Stephen L. W.; Ouis, Ibn-Sina; Vandamme, Peter; Nilsson, Hans-Olof; Ljungh, Asa; Wadstrà ¶m, Torkel Bimal D. M. Theophilus (May 2003) Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence. PCR Mutation Detection Protocols, Methods in Molecular Biology Volume 52, p.765-771. Blaiotta G, Pennacchia C, Ercolini D, Moschetti G, Villani F. (Sep 2003) Combining denaturing gradient gel electrophoresis of 16S rDNA V3 region and 16S-23S rDNA spacer region polymorphism analyses for the identification of staphylococci from Italian fermented sausages. Syst Appl Microbiol. 26(3):423-33 Davies, Charlotte, Katja E. Hill, Katja, Wilson, Melanie, Stephens, Phil, Hill, C. Michael, Harding, Keith and Thomas, David (Aug 2004) Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers Journal of Clinical Microbiology, Vol. 42, No. 8, p. 3549-3557 de Oliveira, Valà ©ria,à Manfio, Gilson, Heitor Luiz da Costa Coutinho, Heitor Keijzer-Wolters, Anneke and van Elsas, Jan. (Apr 2006)Ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil Applied and Environmental Microbiology, Vol 72, No. 4 p. 2756-2764 Ercolini D, Hill PJ, Dodd CE. (Jun 2003) Bacterial community structure and location in Stilton cheese.à Appl Environ Microbiol.;69(6):3540-8. Gast, Rebecca J., Dennett, Mark and Caron, David (Apr 2004) Characterization of Protistan Assemblages in the Ross Sea, Antarctica, by Denaturing Gradient Gel Electrophoresis Applied and Environmental Microbiology, Vol 70, No. 4. p. 2028-2037 Holben, Willam,à Feris, Kevin, Kettunen, Anu and Apajalahti, Juha. (Apr 2004) GC Fractionation Enhances Microbial Community Diversity Assessment and Detection of Minority Populations of Bacteria by Denaturing Gradient Gel Electrophoresis. Applied and Environmental Microbiology, Vol 70, No. 4 p. 2263-2270 Jeroen H. Roelfsema and Dorien J. M. Peters (2005), Denaturing Gradient Gel Electrophoresis (DGGE), Medical Biomethods Handbook, p.79-85 Maukonen, Johanna, Mà ¤ttà ¶, Jaana, Satokari, Reetta,à Sà ¶derlund, Hans, Mattila-Sandholm, Tiina and Saarela, Maria (2006) PCR DGGE and RT-PCR DGGE show diversity and short-term temporal stability in the Clostridium coccoidesââ¬âEubacterium rectale group in the human intestinal microbiota. FEMS Microbiology Ecology (Online early). Miambi E, Guyot JP, Ampe F. (Apr 2003) Identification, isolation and quantification of representative bacteria from fermented cassava dough using an integrated approach of culture-dependent and culture-independent methods. Int J Food Microbiol. 25;82(2):111-20. Mumm S, Jones J, Finnegan P, Henthorn PS, Podgornik MN, Whyte MP. à (Feb. 2002) Denaturing gradient gel electrophoresis analysis of the tissue nonspecific alkaline phosphatase isoenzyme gene in hypophosphatasia. Mol Genet Metab. 75(2):143-53. Neufeld, Josh and Mohn, William. (Aug 2005) Fluorophore-Labeled Primers Improve the Sensitivity, Versatility, and Normalization of Denaturing Gradient Gel Electrophoresis Applied and Environmental Microbiology, Vol.71, No. 8 p. 4893-4896 Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP. (Jun 2001) Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol. 2001 Jun;67(6):2578-85 Yu, Zhongtang and Morrison, Mark. (Aug 2004)Comparisons of Different Hypervariable Regions of rrs Genes for Use in Fingerprinting of Microbial Communities by PCR-Denaturing Gradient Gel Electrophoresis. Applied and Environmental Microbiology, August 2004, p. 4800-4806, Vol. 70, No. 8
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